ANZBMS 2017 レポート
李 智媛（愛媛大学 プロテオサイエンスセンターバイオイメージング部門）
First of all, I wish to acknowledge the travel grant from the JSBMR providing the great opportunity allowing me to attend the joint meeting of ANZBMS-IFMRS, which was held at Brisbane convention center, Australia, over June 17 to 21, 2017. The research topics ranged from musculoskeletal research, clinical and basic science, and the translation of science to health care and clinical practice, all the way to the roles of bone in disease. To fit to their concept of meeting, each day started from exercise sessions (Yoga, Zumba and Pilates) in the beautiful Southbank Parklands on the edge of the river.
There were about 110 posters. I also took part in poster presentation. Although it was not big scale meeting, considering gathering the advantage of gaining lots of feedbacks and ideas from wide audience, I prefer this scale more. There were more than 5 scientists stopped by my poster and gave me quite a few suggestions. It makes me enrich my experience with more information, knowledge and confidence.
It provided me a good opportunity to interact with many other countries researchers and exchange their finding and learning experiences. And I really enjoyed my days in Brisbane. Thank you again.
Live imaging of bone cells “Visualizing osteoclast dynamics by confocal microscopy”
osteoclast, confocal microscope, bone resorption
- 1. University of Western Australia, Nedlands, Australia
The localization and function osteoclasts has long been appreciated by conventional light microscopy, fluorescence and live imaging of these cells on their native substrates is often hampered by the intrinsic auto-fluorescence of bone and limited depth-of-focus encountered when using wide-field epifluorescent systems. His groups overcome these limitations using by confocal microscopy by virtue of its monochromatic lasers and by focusing the field of illumination and light collected to a single point in the same focal plane. His presentation introduced an overview of the application of confocal microscopy for fluorescence and live cell imaging of bone resorbing osteoclasts.
Proffered papers-Basic science II “Osteoclasts have two resorption modes that differ with respect to resorption orientation, aggressiveness, molecular characteristics and drug sensitivity”
osteoclast, pit resorption, trench resorption
- University of Southern Denmark, Denmark
It is believed that osteoclasts are immobile during bone resorption and move without resorbing to a new resorption site. This explains well how round pits are generated, but not how elongate resorption cavities, trenches, are formed. They revealed that trenches reflect osteoclasts that resorb bone while moving, thus pointing to an additional resorption model. They used time-lapse to monitor live the spatiotemporal generation of resorption events by osteoclasts cultured on bone slices. Compared to pit events, trench events showed properties enabling higher aggressiveness: long duration, high erosion speed and long-distance erosion.